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normal human skin fibroblast hsf cells  (ATCC)


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    ATCC normal human skin fibroblast hsf cells
    Normal Human Skin Fibroblast Hsf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skin+fibroblast+hsf+cells/Human+skin+fibroblast/10__1016_slash_j__mtcomm__2024__111385-103-0-18
    Average 95 stars, based on 476 article reviews
    normal human skin fibroblast hsf cells - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Urazole as an unorthodox functional group for fabrication of anionic hydrogels and ion-exchange materials
    Article Snippet: .. Human skin fibroblast (HSF) cells (ATCC, PCS-201030) were incubated at 37 °C under in humidified atmosphere air with 5% CO2 and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Germany) supplemented with 5% Penicillin-Streptomycin Ampicillin (PSA), 5% l-glutamine, and 10% fetal bovine serum (FBS) in tissue culture flasks (TPP, Germany). .. Cells were harvested Trypsin/D-Hanks solution (0.25 w/v %, Gibco) at reach 90% confluency then seeded at a density of 5 × 103 cells/well in a 96-well plate for incubation 24 h. Cell Viability Assay Cell viability analysis was measured using tetrazolium salt WST1 colorimetric assay after treatment of hydrogel extract on seeded cells in a 96-well plate.

    Cell Culture:

    Article Title: Urazole as an unorthodox functional group for fabrication of anionic hydrogels and ion-exchange materials
    Article Snippet: .. Human skin fibroblast (HSF) cells (ATCC, PCS-201030) were incubated at 37 °C under in humidified atmosphere air with 5% CO2 and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Germany) supplemented with 5% Penicillin-Streptomycin Ampicillin (PSA), 5% l-glutamine, and 10% fetal bovine serum (FBS) in tissue culture flasks (TPP, Germany). .. Cells were harvested Trypsin/D-Hanks solution (0.25 w/v %, Gibco) at reach 90% confluency then seeded at a density of 5 × 103 cells/well in a 96-well plate for incubation 24 h. Cell Viability Assay Cell viability analysis was measured using tetrazolium salt WST1 colorimetric assay after treatment of hydrogel extract on seeded cells in a 96-well plate.

    Article Title: Construction of an IL12 and CXCL11 armed oncolytic herpes simplex virus using the CRISPR/Cas9 system for colon cancer treatment.
    Article Snippet: .. HEK293FT cells, human skin fibroblast (HSF) cells, SW620 cells, MC38 cells and Vero cells were originally from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 12800-017) with 10% FBS at 37 ◦C and 5% CO2. ..

    Article Title: DEVELOPMENT OF FLUCONAZOLE-LOADED POLY(VINYL ALCOHOL)/POLY(ETHYLENE GLYCOL) BLENDED FILM FOR TREATMENT OF CANDIDA-INFECTED CUTANEOUS WOUNDS
    Article Snippet: In this work, we aimed to develop fluconazole (FLU)-loaded polymer blended film (FLU-film) for candidiasis wounds.. The films were prepared from poly(vinyl alcohol) (PVA) and poly(ethylene glycol) 1000 (PEG 1000) mixed at different proportions by weight, casted on a petri dish, and dried at 60oC for 12 h. Tensile strength, swelling ratio, and morphology were analyzed to determine an appropriate film for wound application.. The selected film was loaded with 0.5% w/w FLU, where the drug homogeneity was ascertained.

    Article Title: Construction of an IL12 and CXCL11 armed oncolytic herpes simplex virus using the CRISPR/Cas9 system for colon cancer treatment
    Article Snippet: .. HEK293FT cells, human skin fibroblast (HSF) cells, SW620 cells, MC38 cells and Vero cells were originally from ATCC and cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, 12800-017) with 10% FBS at 37 °C and 5% CO 2 . ..

    Modification:

    Article Title: Urazole as an unorthodox functional group for fabrication of anionic hydrogels and ion-exchange materials
    Article Snippet: .. Human skin fibroblast (HSF) cells (ATCC, PCS-201030) were incubated at 37 °C under in humidified atmosphere air with 5% CO2 and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Germany) supplemented with 5% Penicillin-Streptomycin Ampicillin (PSA), 5% l-glutamine, and 10% fetal bovine serum (FBS) in tissue culture flasks (TPP, Germany). .. Cells were harvested Trypsin/D-Hanks solution (0.25 w/v %, Gibco) at reach 90% confluency then seeded at a density of 5 × 103 cells/well in a 96-well plate for incubation 24 h. Cell Viability Assay Cell viability analysis was measured using tetrazolium salt WST1 colorimetric assay after treatment of hydrogel extract on seeded cells in a 96-well plate.

    Article Title: Construction of an IL12 and CXCL11 armed oncolytic herpes simplex virus using the CRISPR/Cas9 system for colon cancer treatment.
    Article Snippet: .. HEK293FT cells, human skin fibroblast (HSF) cells, SW620 cells, MC38 cells and Vero cells were originally from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 12800-017) with 10% FBS at 37 ◦C and 5% CO2. ..

    Article Title: 2D Material-Based Nanofibrous Membrane for Photothermal Cancer Therapy.
    Article Snippet: One of the clinical challenges facing photothermal cancer therapy is health risks imposed by the photothermal nanoagents in vivo.. Herein, a photothermal therapy (PTT) platform composed of a 2D material-based nanofibrous membrane as the agent to deliver thermal energy to tumors under near-infrared (NIR) light irradiation is described.. The photothermal membrane, which is fabricated by an electrospinning poly(L-lactic acid) (PLLA) nanofibrous membrane loaded with bismuth selenide (Bi2Se3) nanoplates, exhibits very high photothermal conversion efficiency and long-term stability.

    Article Title: DEVELOPMENT OF FLUCONAZOLE-LOADED POLY(VINYL ALCOHOL)/POLY(ETHYLENE GLYCOL) BLENDED FILM FOR TREATMENT OF CANDIDA-INFECTED CUTANEOUS WOUNDS
    Article Snippet: In this work, we aimed to develop fluconazole (FLU)-loaded polymer blended film (FLU-film) for candidiasis wounds.. The films were prepared from poly(vinyl alcohol) (PVA) and poly(ethylene glycol) 1000 (PEG 1000) mixed at different proportions by weight, casted on a petri dish, and dried at 60oC for 12 h. Tensile strength, swelling ratio, and morphology were analyzed to determine an appropriate film for wound application.. The selected film was loaded with 0.5% w/w FLU, where the drug homogeneity was ascertained.

    Article Title: Construction of an IL12 and CXCL11 armed oncolytic herpes simplex virus using the CRISPR/Cas9 system for colon cancer treatment
    Article Snippet: .. HEK293FT cells, human skin fibroblast (HSF) cells, SW620 cells, MC38 cells and Vero cells were originally from ATCC and cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, 12800-017) with 10% FBS at 37 °C and 5% CO 2 . ..



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